Identification of silencing suppressors of potato virus M
نویسندگان
چکیده
Gene silencing is an important regulatory and defense mechanism in plants. Plant viruses evolved some proteins that suppress cellular RNA interference mechanism. In this work identification of silencing suppressors of potato virus M (PVM) is reported. The cDNA sequences of different PVM open reading frames were cloned into the Gateway-compatible binary T-DNA destination vector pMDC32, harboring a double 35S promoter. Each of these constructs was electroporated into Agrobacterium tumefaciens C58C1 which then were agroinfiltrated into Nicotiana benthamiana 16C line. It was shown that viral coat protein (34K) as well as some other PVM proteins (12K, 11K) possessed suppressor properties of cellular silencing activity.
منابع مشابه
A dual strategy for the suppression of host antiviral silencing: two distinct suppressors for viral replication and viral movement encoded by potato virus M.
Viruses encode RNA silencing suppressors to counteract host antiviral silencing. In this study, we analyzed the suppressors encoded by potato virus M (PVM), a member of the genus Carlavirus. In the conventional green fluorescent protein transient coexpression assay, the cysteine-rich protein (CRP) of PVM inhibited both local and systemic silencing, whereas the triple gene block protein 1 (TGBp1...
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